مشخصات پژوهش

عنوان	A novel highly sensitive compilation-detachment fluorescence sensing strategy based on RNA-cleavage DNAzyme for MDA-MB-231 breast cancer biomarker determination
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Biosensor, Syber Green, dsDNA, RNA-Cleavage DNAzyme, MDA-MB-231, Cell line, Magneto sensor, Fluorescence
چکیده	Herein, we designed a novel and highly sensitive fluorescence multicomponent detachable platform for MDA-MB-231 breast cancer cell detection as a model. The RNA cleavage DNAzyme was used as a central operator of the multicomponent probe through which compilation and induced detachment of probe was done. During the compilation step, the dsDNA-Sybr green 1 complexes on gold nanoparticles (GNP@dsDNA@SG1) were assembled. The intercalated Sybr green in the DNA structure has been used as an amplified signal generator on one site of DNAzyme and magnetic nanoparticles (MNP) act as a biological carrier and probe collector on the opposite side. The enzyme activator co-factor (MDA-MB- 231 cell cytoplasmic protein) provokes the activation of the catalytic core of enzyme sequence in the DNAzyme molecule, followed by cleavage reaction in the substrate sequence and releasing GNP@ dsDNA@SG1 into the solution. The results indicate that the Sybr green emission fluorescence (520 nm) increases with the increment of MDA-MB-231 protein concentration in the linear dynamic range of 8.10  102 to 1.95 ng ml1 (0.77  103-0.019 cell ml1) with a detection limit (LOD) of 1/72  102 pg ml1 under optimal conditions. The proposed immunosensor has great potential in developing ultrasensitive and rapid diagnostic platforms.
پژوهشگران	فرنوش خسروبخش (نفر چهارم)، عبدالله سلیمی (نفر سوم)، رحمان حلاج (نفر دوم)، ژاله غفاری (نفر اول)