مشخصات پژوهش

عنوان	Layer by layer assembly of catalase and amine-terminated ionic liquid onto titanium nitride nanoparticles modified glassy carbon electrode: Study of direct voltammetry and bioelectrocatalytic activity
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Layer by layer, Catalase, IL, Titanium nitride,nanoparticle,H2O2, Biosensor
چکیده	A novel, simple and facile layer by layer (LBL) approach is used for modification of glassy carbon (GC) electrode with multilayer of catalase and nanocomposite containing 1-(3-Aminopropyl)-3-methylimidazolium bromide (amine terminated ionic liquid (NH2-IL)) and titanium nitride nanoparticles (TiNnp). First a thin layer of NH2-IL is covalently attached to GC/TiNnp electrode using electro-oxidation method. Then, with alternative self assemble positively charged NH2-IL and negatively charged catalase a sensitive H2O2biosensor is constructed, whose response is directly correlated to the number of bilay-ers. The surface coverage of active catalase per bilayer, heterogeneous electron transfer rate constant (ks) and Michaelis–Menten constant (KM) of immobilized catalase were 3.32 × 10−12 mol cm−2, 5.28 s−1 and 1.1 mM, respectively. The biosensor shows good stability, high reproducibility, long life-time, and fast amperometric response with the high sensitivity of 380 A mM−1cm- 2 .and low detection limit of 100 nM at concentration range up to 2.1 mM
پژوهشگران	امین رستمی (نفر چهارم)، عبدالله سلیمی (نفر دوم)، رحمان حلاج (نفر سوم)، شقایق سعادتی (نفر اول)