مشخصات پژوهش

عنوان	Magnetoimmunosensor for simultaneous electrochemical detection of carcinoembryonic antigen and α-fetoprotein using multifunctionalized Au nanotags
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Electrochemical, Immunosensor, Simultaneous detection, CEA and AFP, Thionine, Ferrocene, Au nanotags.
چکیده	We report a sandwich-type electrochemical immunoassay for simultaneous determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP), using multifunctionalized gold nanoparticles as signal tags. Two nanotags including thionine-Au nanoparticles and ferrocene-Au nanoparticles were synthesized and used as distinguishable indicators. The sensor was fabricated by means of co-immobilization of primary anti-CEA (AbCEA) and anti-AFP (AbAFP) antibodies on the surface of Fe3O4 nanoparticle, the strategy is based on observation distinct voltammetric peak derived from distinguish probe (AbCEA-Au-thionine and AbAFP-Au -ferrocene nanotages). Experimental results revealed that this immunosensor enabled simultaneous determination of CEA and AFP in the linear ranges of (0.05- 120 ngmL-1) and (0.05-100 ngmL-1), respectively. The detection limits (DL) for AFP and CEA were 0.012 ngmL-1 and 0.018 ngmL-1, respectively (S/N=3). Furthermore, the methodology was also evaluated for the analysis of AFP and CEA in the clinical human serum specimens, the obtained results was in desirable agreement with clinical data.
پژوهشگران	رحمان حلاج (نفر سوم)، عبدالله سلیمی (نفر دوم)، نگار علیزاده (نفر اول)