مشخصات پژوهش

عنوان	Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme as Electrocatalysts for Sensitive Electrochemical Immunosensing of HBsAg
نوع پژوهش	مقاله ارائه شده کنفرانسی
کلیدواژه‌ها	Immunosensor, Hemin/G-Quadruplex, Fe3O4 Nanoparticle, DNAzyme, HBsAg
چکیده	Here we prepared an electrochemical immunosensor employing Au sheet as working electrode, Fe3O4 nanoparticle as supporting matrix and hemin/G-quadruplex DNAzyme as signal amplifier for determination of HBsAg. Fe3O4 nanoparticle was used for immobilization of primary antibody and protein target was sandwiched between magnetic nanoparticle and AuNP-hemin/G-quadruplex which was attached to methylene blue. Hemin/G-quadruplex structure as HRP mimicking-DNAzyme significantly improved the catalysis of H2O2 by oxidation of methylene blue. Experimental results revealed that the immunoassay enabled the determination of HBsAg with linear working ranges of 1–100 pgmL_1. The limit of detection for HBsAg is 0.46 pgmL_1.. The immunosensor exhibited high sensitivity and highly efficient catalysis of DNAzyme, showing great promise for clinical application and providing new opportunities for fast disease diagnosis.
پژوهشگران	رحمان حلاج (نفر سوم)، عبدالله سلیمی (نفر دوم)، نگار علیزاده (نفر اول)