مشخصات پژوهش

عنوان	Spatial modulation and abiotic elicitors responses of the biosynthesis related genes of mono/triterpenes in black cumin (Nigella sativa
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Black cumin,Mevalonic acid,2-C-methylerythritol 4-phosphate,Rate limiting,Semi quantitative PCR,Terpene biosynthesisa
چکیده	Black cumin (Nigella sativa) is a medicinal herb belongs to the Ranunculacea which raised attention dueto its pharmaceutical properties. Medical significance of N. sativa mainly attributed to its oxygenated monoterpenes and triterpene saponins which are biosynthesized via the methyl erythritol phosphate(MEP) and MVA (mevalonic acid) pathways, respectively. In order to improve our understanding of monoterpene and triterpene metabolism the partial sequence of a hypothetical monoterpene synthase(MTS) was isolated from N. sativa for the first time and subsequently the relative gene expression of twogenes of monoterpene metabolism including geranyl diphosphate synthase (GDS), the isolated monoter-pene synthase (MTS) and two genes of triterpene saponins biosynthesis including squalene epoxidase(SQE) and - amyrin synthase (ˇAS) were monitored in response to two universal abiotic elicitors including methyl jasmonate (MJ) and salicylic acid (SA), also in different tissues of N. sativa includingleaves, stems, roots, capsules and immature seeds. Results of semi quantitative reverse transcription PCRrevealed differential regulation of these genes in N. sativa tissues and also in response to abiotic elicitors.Each gene followed a different expression profile and expression level in target tissues. The expression of all studied genes were up-regulated by exogenous application of MJ and SA on N. sativa leaves (at 24 hafter treatments), except of GDS which its transcript was not induced by SA. The higher expression of these genes in response to abiotic elicitors might reflect an elevation in the corresponding metabolites;hence elicitation is an applicable tool for enhancement of pharmaceutically active mono/triterpenes in N. sativa.
پژوهشگران	قادر میرزاقادری (نفر چهارم)، بهمن بهرام نژاد (نفر سوم)، محمد مجدی (نفر دوم)، ریزان الیاسی (نفر اول)