مشخصات پژوهش

عنوان	Gene expression of calcium/calmodulin-dependent protein kinase IIα decreased in rat midbrain after induction of morphine analgesic tolerance
نوع پژوهش	مقاله ارائه شده کنفرانسی
کلیدواژه‌ها	Gene expression, Midbrain, Morphine tolerance, Analgesia
چکیده	Background and Aim: Morphine is widely used to treat chronic pain but its utility is hindered by the development of tolerance to its analgesic effects.Pharmacological data have shown that calcium/calmodulin-dependent protein kinase IIα (CamKIIα) is involved in morphine-induced analgesic tolerance.The aim of this study was to examine changes in gene expression of CamKIIα during and after induction of morphine analgesic tolerance. Methods: We used male Wistar rats weighing 280-320g in these experiments. Two groups of rats (n= 8) received saline 1 ml/kg or morphine 10 mg/kgtwice daily for a schedule of 7 days. Morphine induced analgesic tolerance was assessed usinga hotplate test on days 1, 4 and 8 (one day after the last injection). Rat midbrain was also extracted in separate experimental groups on days 4 and 8 of the schedule.Change in gene expression of CamKIIα was examined with a semi-quantitative RT-PCR method. Results: First, the result of hotplate test showed that repeated injections of morphine induced analgesictolerance on day 8 but there was also a significant decrease in morphine analgesia on day 4 of the schedule. Second, the result of gene expression for CamKIIα in rat midbrain on day 4 showed an increase but it significantly decreased on day 8 after induction of morphine analgesic tolerance. Conclusion: It can be concluded that changes in gene expression of CamKIIα in rat midbrain may underlie morphine-induced analgesic tolerance.
پژوهشگران	جلال رستم زاده (نفر سوم)، شمس الدین احمدی (نفر دوم)، اسرین رشیدی (نفر اول)