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چکیده
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AN EFFICIENT METHOD was developed for organogenic plant regeneration of Cercis siliquastrum through callus cultures. The leaves as well as stems of in vitro grown seedlings were cultured on Murashige and Skoog (MS) medium containing 3% sucrose, 0.8% agar, and different concentrations of 2,4-Dichlorophenoxyacetc acid (2,4-D) and naphthalene acetic acid (NAA) in combination with 4.43 μM benzyl adenine (BA). The highest frequency of white, compact and nodular calli (48%) was induced from leaf explants cultured on MS medium containing 9.04 μM 2,4-D and 4.43 μM benzyl adenine (BA). According to calli weighing, however, the most callus growth and proliferation (2.4 gram) was achieved on MS medium supplemented with 18.09 μM 2,4-D and 4.43 μM benzyl adenine (BA). Shoot regeneration was occurred when the nodular calli were transferred onto MS medium supplemented with 8.87 μM BA and 4.54 μM thidiazorun (TDZ). Addition of 5.77 μM GA3 along with 4.43 μM BA to the medium promoted the shoot elongation. For rooting experiments, the regenerated shoots were transferred onto MS medium containing different concentrations of IBA alone, or in combination with kinetin (KIN). The plants were acclimatized and successfully transferred to soil under greenhouse conditions.
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