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عنوان
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Egg Yolk Antibody Response to Heat or Formalin Treatment of E. coli O157:H7 in Laying Hens
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نوع پژوهش
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مقاله ارائه شده کنفرانسی
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کلیدواژهها
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IgY, E. coli O157:H7, heat killed, formalin, ammonium sulfate, gel chromatography)
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چکیده
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Escherichia coli O157:H7, heated at 121C for 20 min or treated with Formalin, was injected into laying hens to prepare hyperimmune eggs. Chicken egg yolk antibody (IgY) raised against E. coli O157:H7 treated by heat or formalin was found in highly specific activity levels by ELISA. E. coli O157:H7-specific IgY extracted by ammonium sulfate precipitation and gel chromatography on Sephacryl S-300 of the egg yolk water-soluble fraction contained 15.5 mg/g of total IgY % in heat killed and 10.0 mg % in formalin killed of specific IgY. Anti-Heat killed IgY cross-reacted 55.3% with formalin killed E. coli. The cross-reactivity of anti-Formalin killed IgY with heat killed E. coli was 42.4%.
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پژوهشگران
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جونگ سیم (نفر سوم)، امیرعلی صادقی (نفر دوم)، چ.چ سانوو (نفر اول)
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