مشخصات پژوهش

عنوان	Ectopically expressed leaf and bulb lectins from garlic (Allium sativum L.) protect transgenic tobacco plants against cotton leafworm (Spodoptera littoralis)
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Allium sativum leaf lectin (ASAL), Allium sativum bulb lectin (ASAII), lectin, Lepidoptera, Spodoptera littoralis, transgenic tobacco plants.
چکیده	The insecticidal activity of the leaf (ASAL) and bulb (ASAII) agglutinins from Allium sativum L. (garlic) against the cotton leafworm, Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae) was studied using transgenic tobacco plants expressing the lectins under the control of the constitutive CaMV35S promoter. PCR analysis confirmed that the garlic lectin genes were integrated into the plant genome. Western blots and semi-quantitative agglutination assays revealed lectin expression at various levels in the transgenic lines. Biochemical analyses indicated that the recombinant ASAL and ASAII are indistinguishable from the native garlic lectins. For ASAL and ASAII, the expression levels in tobacco leaves were approximately 10 and 20 µg/g leaf tissue, respectively. Insect bioassays using detached leaves from transgenic tobacco plants demonstrated that the ectopically expressed ASAL and ASAII significantly (p<0.05) reduced the weight gain of 4th instar larvae of S. littoralis. Further on, the lectins retarded the development of the larvae and their metamorphosis, and were detrimental to the pupal stage resulting in weight reduction and lethal abnormalities. Total mortality was scored with ASAL compared to 60% mortality with ASAII. These findings suggest that garlic lectins are suitable candidate insect resistance proteins for the control of S. littoralis and perhaps other insects through a transgenic approach.
پژوهشگران	امین صادقی (نفر اول)، سیلویا برویدرس (نفر سوم)، ویلی ج.پیومانس (نفر ششم به بعد)، هنری دگریو (نفر پنجم)، گی سماخه (نفر دوم)، ژان پیر هیرنالیستین (نفر چهارم)