مشخصات پژوهش

عنوان	C-banded karyotype of Thinopyrum bessarabicum and identification of its chromosomes in wheat background
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	C-banding, Thinopyrum bessarabicum, Tritipyrum, wheat
چکیده	C-banded pattern in two accessions of Thinopyrum bessarabicum (Save ex Rayss) A Löve (2n=2x=14, EbEb) and their idiogram was established. C-banding analysis was further used to identify the chromosomes of Tritipyrum amphiploid (2n=6x=42, AABBEbEb) and a BC1F2 genotype from wheat and Tritipyrum. Two 18S-26S rDNA loci were detected on Th. bessarabicum chromosomes by in situ hybridization using an 18S-26S rDNA probe. Eb chromosomes in Tritipyrum generally were identified by their distinctive C-banding patterns which reflected heterochromatin regions. C-banding procedure resulted in sharp and distinct bands in one or both ends of Eb chromosomes without interval bands. Observed C-bands in Eb genome mainly reflected the telomeric and subtelomeric sequences which also showed more strong signals in genomic in situ hybridization. Results showed the importance of the C-banding technique as a screening tool in identification of addition and substitution lines in the progenies of wheat and Tritipyrum crosses during segregating generations
پژوهشگران	قاسم کریم زاده (نفر سوم)، حسین شاهسوند حسنی (نفر دوم)، قادر میرزاقادری (نفر اول)