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چکیده
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A novel “on-off-on” fluorescent sensor was developed for the sensitive and selective detection of glutathione (GSH) based on a Forster-resonance energy transfer (FRET) mechanism between a dual-ligand zirconium-based metal-organic framework (Zr-MOF) and manganese dioxide (MnO2) nanoparticles. The dual-ligand (H3TATAB and NH2-BDC) synthesized Zr-MOF served as a good fluorophore, while the MnO2 nanoparticle was an effective quencher. GSH presence causes the reduction of MnO2 to Mn2+, which suppresses the FRET process and thus triggers the recovery of quenched fluorescence. This sensor shows excellent performance with wide linear range (5–110 μM), low detection limit of 1.85 μM, and satisfactory selectivity toward potential interfering compounds. The utility of the sensor in actual samples was effectively confirmed by the determination of GSH in human serum samples with satisfactory recoveries (94.82–102.35%) and (96.55–101.49%), indicating its great promise as a clinical diagnostic aid. These results collectively illustrate that Zr-MOF5/MnO2 nanocomposite is a good and highly prospective sensor for GSH with a high prospect of making significant bioanalytical chemistry and biomedical diagnosis breakthroughs.
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