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چکیده
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Probiotics are living and non-pathogenic microorganisms that are part of the intestinal microbial flora. Probiotics are composed of different species, the most important of which are Lactobacillus and Bifidobacterium. The aim of this study is to investigate the probiotic potential of Lactobacillus isolated from Markhoz goat feces [1,2]. First, freshly prepared feces were collected through a sterile strainer. After centrifuging the liquid passed through the filter at 3000 rpm for 2 minutes, the liquid was inoculated in MRS broth culture medium. Then the samples were incubated for 24 hours at 35°C. Then, this medium was sub-cultured on MRS agar. Grown colonies were used to perform diagnostic microbial tests including Gram staining, catalase test and fermentation of carbohydrates. Finally, for the final confirmation of the suspected Lactobacillus bacteria, the spices-specific PCR and subsequently gene sequencing was applied. The obtained sequence was identified in the NCBI database as Lactiplantibacillus plantarum. After isolating the bacteria, its probiotic activity including bile tolerance, salt tolerance (3 and 6%), pH tolerance: 2.5 for 2 hours, antimicrobial properties against Escherichia coli, Salmonella typhimurium and Staphylococcus aureus were confirmed. The present study showed that the feces of native goats of Kurdistan province (Markhoz goat) contain Lactobacillus bacteria that have high probiotic power and these bacteria can be used in the design a new drug, especially in the field of fighting against antibiotic resistant pathogens.
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