مشخصات پژوهش

عنوان	Repetitive DNA landscape in essential A and supernumerary B chromosomes of Festuca pratensis Huds
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Tandem repeats, Satellite DNA, Next Generation Sequencing
چکیده	Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (−B) and B chromosome containing (+B) individuals of F. pratensis. 61% of the nuclear genome is composed of repetitive sequences. 43.1% of the genome are transposons of which DNA transposons and retrotransposons made up 2.3% and 40.8%, respectively. LTR retrotransposons are the most abundant mobile elements and contribute to 40.7% of the genome and divided into Ty3-gypsy and Ty1-copia super families with 32.97% and 7.78% of the genome, respectively. Eighteen different satellite repeats were identified making up 3.9% of the genome. Five satellite repeats were used as cytological markers for chromosome identification and genome analysis in the genus Festuca. Four satellite repeats were identified on B chromosomes among which Fp-Sat48 and Fp-Sat253 were specific to the B chromosome of F. pratensis.
پژوهشگران	قادر میرزاقادری (نفر سوم)، آندریاس هوبن (نفر دوم)، رحمان ابراهیم زاده گان (نفر اول)