مشخصات پژوهش

عنوان	Amine-functionalized graphene as an effective electrochemical platform toward easily miRNA hybridization detection
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Amine functionalized reduced graphene oxide Electrochemical platform MicroRNA detection microRNA-155
چکیده	Graphene has a superior potential as a preferable platform in sensing and catalysis application. Here, we proposed amino-graphene as a straightforward platform for immobilization of biomolecules toward sensing of cancer diagnostic. MicroRNA-155 is selected, since it is known to play an important role in many cancer diagnostics. The process of sensor fabrication is simple and includes of two steps: (1) preparation of amino-graphene and assembling of it on the surface of electrode, (2) attaching of antimicroRNA- 155 into amino-graphene through crosslinking by glutaraldehyde. Different steps of biosensor fabrication are characterized by atomic force microscopy and electrochemical impedance spectroscopy, and hybridization of target microRNA-155 with its complementary is assayed by differential pulse voltammetry. Hence, biosensor is able to detect the miRNA-155 at femtomolar concentration level and the proposed platform can be simply applicable for immobilization of different biomolecules for numerous applications.
پژوهشگران	ئاسو نوایی (نفر سوم)، بیگرد کاوسی (نفر دوم)، عبدالله سلیمی (نفر اول)