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چکیده
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The objective of this study was to determine the effects of genistein or 17𝛃-estradiol (E2) on the reproductive physiology in male gibel carp, Carassius auratus gibelio.Maturingmale gibel carp received intraperitoneal injections of E2 (10 𝛍g/g body weight), one of two genistein doses (5 𝛍g/g body weight, G5, or 50 𝛍g/g body weight, G50), or the injection vehicle every other day for 10 d. Disruptions in reproductive capacity were determined by measuring indices of sperm quality, plasma metabolites and sex steroids, histological analyses of testes, fertilization rate, and offspring viability. E2 and genistein treatment reduced gonadosomatic index and milt volume, while reduction in spermatozoa concentration and spermatocrit occurred only in E2 and G50-treated males. Histological examination of the testes indicates that E2 and genistein inhibited reproductive capacity through disruption of the spermatogenesis in males. Genistein reduced fertilization rate and offspring viability at 6 d after hatch (d.a.h.). Plasma testosterone and E2 decreased and increased, respectively, with E2 and G50 treatment. E2 and G50 treatment altered plasma metabolite phosphorus, calcium, cholesterol, and triglyceride. These findings indicate that genistein can negatively affect reproductive capacity in male gibel carp, suggesting that high dietary genistein may impair gonad development.
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