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چکیده
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Pear fruit set was affected by several genetic and environmental factors including incompatibility. Self-incompatibility (SI) is controlled in Rosaceae by a homomorphic, gametophytic, monofactorial, multiallelic incompatibility system and pollen rejection occurs in the style. The incompatibility locus controls self-incompatibility as well as intra-specific cross-incompatibility. The present research was carried out during 2004 growing season at Tarbiat Modares University (TMU) Iran, in order to evaluate incompatibility in five European pear (Pyrus communis L.) cultivars. Polymerase chain reaction (PCR) with specific primers was applied to investigate SI genes in pear cultivars including `Shah-Miveh’, `Spadona’, `Sar-drood’, `Mohammad-Ali’ and `Bartlet’. DNA was extracted according to the modified CTAB method and SI gene was amplified using specific primers. The results indicated that primers could amplify the SI gene and that a S-locus existed in these cultivars. The first pair of primers showed that there was one pair of S1, S3-S7 alleles in ‘Shah-Miveh’ and ‘Mohammad-Ali’. The similar band pattern with S2 allele was observed in ‘Sard-Rod’ and ‘Bartlet’ cultivars. In addition, in ‘Spadona’, ‘Sard-Rod’ and ‘Bartlet’ a 1400 base pair band was amplified suggesting the existence of a possible new allele. Similar results were observed using other primers. In conclusion, we suggest using a suitable combination of cultivars with different S alleles for newly established pear orchards and breeding programs.
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