مشخصات پژوهش

عنوان	Survey for Grapevine fanleaf virus in vineyards of north-west Iran and genetic diversity of isolates in the coat protein
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	certification scheme, phylogenetic analysis
چکیده	Grapevine fanleaf virus (genus Nepovirus) was detected by double antibody sandwich enzyme-linked immunosorbant assay (DAS-ELISA) in 31 of 134 grapevine samples from the north-west of Iran. Reverse transcriptase polymerase chain reaction with two primer sets (S2515/A3300 and CP433V/912C) targeting the Grapevine fanleaf virus coat protein gene resulted in amplification of the expected DNA fragments from some but not all DAS-ELISA positive samples. At the nucleotide level, the Iranian isolates were 84–95% identical to each other and 84–91% identical to other isolates from around theworld. This is the first comprehensive analysis of the distribution and genetic diversity of Grapevine fanleaf virus in Iran.
پژوهشگران	محمد حاجی زاده (نفر دوم)، نعمت سخندان بشیر (نفر اول)