مشخصات پژوهش

عنوان	Caspase-dependent apoptosis induced by two synthetic halogenated flavanones, 3',7-dichloroflavanone and 3',6-dichloroflavanone, on human breast and prostate cancer cells
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Apoptosis; flavanone; prostate cancer; breast cancer
چکیده	The destruction of cancer cells with chemotherapeutic agents is normally achieved through apoptosis. We previously introduced two synthetic halogenated flavanones derivatives, 3',7-dichloroflavanone (3'-7 DCF) and 3',6-dichloroflavanone (3'-6 DCF) as potential apoptosis-inducing agents. In the current study we investigated the ability of these compounds in triggering intrinsic or/and extrinsic pathway of apoptosis in breast and prostate cancer cells. Also the synergistic effect of 3'-7 DCF with TLR3 (Toll-like receptor 3) agonist in apoptosis induction was evaluated on PC3 and LNCaP human prostate cancer cells. The involved pathway of apoptosis in the treated cells was delineated by caspase-3 activity assay, PARP-1 (poly(ADP-ribose)polymerase-1) cleavage, and procaspase-9 cleavage as markers of the intrinsic pathway and procaspase-8 cleavage as the marker of the extrinsic pathway. With the exception of the normal cells, treatment of the all cell lines with both 3'-7 DCF and 3'-6 DCF triggered the cleavage of procaspase-8 and -9. These results indicate that the intrinsic and the extrinsic pathways of apoptosis are mechanism of the toxicity of flavanones in these cancer cell lines. However, the cytoxicity of the compound 3'-7 DCF was not synergistic with TLR3 agonist. Interestingly, the activation of caspases-9 preceeded that of caspase-8 suggesting that the intrinsic pathway is the primary reason for apoptosis induction by the flavanones.
پژوهشگران	علیرضا فرومدی (نفر ششم به بعد)، سهیلا اژدری (نفر پنجم)، جمشید داودی (نفر چهارم)، سوسن کبودانیان اردستانی (نفر سوم)، ملیحه صفوی (نفر اول)، راحله شاکری (نفر دوم)