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چکیده
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A novel method for simple and sensitive determination of glucose based on the peroxyoxalate chemiluminescence (PO-CL) detection of enzymatically generated H2O2 was investigated. Influence of various experimental parameters on glucose sensing, including the action time of the enzyme, solution pH, interferents and the concentration of CL reagents was investigated. Under the optimum condition, the linear response range of glucose was found to be 2.50 × 10−6 to 1.75 × 10−4 mol/L, and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) was 1.10 × 10−6 mol/L. The present method has been used to determine the glucose concentrations in real serum and urine samples with satisfactory results.
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