Emmer wheat cultivation in Iran has been confined to the north-east villages of the country. 13 landraces in these regions was collectedand their genetic diversity was investigated along with 42 genotypes of domesticated emmer wheat of other countries of Fertile Crescent. Start codon targeted (SCoT) markers was used during this study. All 10 primers generated a total of 89 fragments, with a mean of 8.9 ranging from 6 to 12 per primer. Of 89 bands, 47 (52.22%) were polymorphic. A high level of gene diversity index was observed. Across the 10 primers, polymorphic information content (PIC) ranged from (0.094) to (0.21), with a mean of 0.13. The results will provide much more useful information for the management of germplasm and will also be useful to improve the current breeding strategies.