The purpose of this study was to investigate the effects of Zinc oxide nanoparticles on some hematological parameters and serological enzymes in Japanese quail .Seventy five Japanese quail in a completely randomized design with 5 treatments for 42 days (levels ppm 10, 30, 90, 0 nanoparticles of zinc oxide and zinc 30 ppm common form of zinc in diet) and 5 replicate groups of male quail (each repeated three birds per cage) were investigated. The parameters evaluated, were red blood cells, white blood cells, hematocrit, hemoglobin, the mean corpuscular volume (MCV), the mean corpuscular hemoglobin (MCH) , the mean corpuscular hemoglobin concentration (MCHC), serum cholesterol, triglycerides, glucose, total bilirubin, direct bilirubin, calcium, phosphorous, iron, zinc concentration, serum levels of total protein, Alkaline phosphatase (ALP), Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT). Observations using SAS software and statistical analysis using GLM ANOVA and Duncan are multiple range tests were used to compare means. There were no significant differences (p>0/05) between treatments in hematocrit and White blood cells, MCHC, triglycerides, glucose, total bilirubin, direct bilirubin, phosphorous concentrations in the serum. The red blood cell, hemoglobin, the serum concentration of calcium and Iron were significantly decreased (p<0/05), but MCV and MCH were significantly increased (p<0/05). The serum concentration of cholesterol and zinc were significantly (p<0/05) higher in birds fed diets containing nanoparticles than those of the control groups. Total protein concentration in the serum were decreased significantly (p<0/05) in the birds fed 30 ppm of zinc oxide nanoparticles. Although the AST activity was significantly higher (p<0/05) in control groups than those of the other groups, but there weren't significant difference in ALP and ALT activities between all groups. These results suggest that the common form of zinc can be replaced by zinc
