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Title
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Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme as Electrocatalysts for Sensitive Electrochemical Immunosensing of HBsAg
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Type
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Presentation
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Keywords
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Immunosensor, Hemin/G-Quadruplex, Fe3O4 Nanoparticle, DNAzyme, HBsAg
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Abstract
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Here we prepared an electrochemical immunosensor employing Au sheet as working electrode, Fe3O4 nanoparticle as supporting matrix and hemin/G-quadruplex DNAzyme as signal amplifier for determination of HBsAg. Fe3O4 nanoparticle was used for immobilization of primary antibody and protein target was sandwiched between magnetic nanoparticle and AuNP-hemin/G-quadruplex which was attached to methylene blue. Hemin/G-quadruplex structure as HRP mimicking-DNAzyme significantly improved the catalysis of H2O2 by oxidation of methylene blue. Experimental results revealed that the immunoassay enabled the determination of HBsAg with linear working ranges of 1–100 pgmL_1. The limit of detection for HBsAg is 0.46 pgmL_1.. The immunosensor exhibited high sensitivity and highly efficient catalysis of DNAzyme, showing great promise for clinical application and providing new opportunities for fast disease diagnosis.
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Researchers
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Rahman Hallaj (Third Researcher), Abdollah Salimi (Second Researcher), negar Alizadeh (First Researcher)
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