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Title
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Preparation and in vitro characterization of retinoic acid-loaded poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) micelles
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Type
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JournalPaper
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Keywords
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Poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone), Micelle, All trans retinoic acid ,ATRA), H1299, Cytotoxicity)
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Abstract
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In order to achieve the controlled release of all-trans-retinoic acid (ATRA), poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) copolymer with average molecular weight of 5.34 kDa was synthesized. The nanosized micelles were prepared from copolymer by nano-precipitation method. Critical association concentration (CAC) of micelles was measured by fluorimetry and results indicated low CAC value of micelles (1.9 × 10-3 g/L). ATRA was encapsulated in the core of micelles using different ratios of drug to copolymer. In the case of 10% drug to polymer ratio, more than 80% of the drug was released within 3 days, whereas for ratio of 2% more than 90% of the drug was released within 3 h. The cytotoxic study performed by MTT assay showed that H1299 survival percent decreased significantly (P ≤ 0.05) after exposure to drug-loaded micelles, while no proliferation inhibition effect was observed by either free ATRA or blank PCL-PEG-PCL micelles.
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Researchers
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Ali Fattahi (Fifth Researcher), Marziyeh Hajialyani (Third Researcher), Saeedeh Khazaei (Second Researcher), Ebrahim Shakiba (First Researcher), Bandar Astinchap (Fourth Researcher)
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