The current research investigates the regional differences in benefiting from innovation toward growth ambitions in a sample of female entrepreneurs in the Middle East and North Africa (MENA) and Nordic region. A sample of 4,048 early-stage female entrepreneurs from these two regions who were participated in annual surveys of Global Entrepreneurship Monitor was selected for further analysis. The results of data analysis using hierarchical linear modeling (mixed models) revealed that innovation benefits growth ambitions of early-stage female entrepreneurs. Further, the regional differences affect the benefit of innovation in shaping growth ambitions, so that, in Nordic region, the early-stage female entrepreneurs expect more growth out of their innovation as compared with their counterparts in MENA region. These results have been discussed regarding to existing literature, and future research directions have been suggested based on the results.