In recent years, organic system for cultivation medicinal and aromatic plants has been a growing movement. In present research we compared the effect of organic and chemical system on growth and yield of Anise (pimpinella anisum L.). Field experiment was conducted in the agriculture research station, at Sanandaj, Iran, in 2008. Experimental factors of organic system were vermicompost, PGPR (inoculation and not-inoculation) and zeolite. The treatments were arranged as factorial in a randomized complete blocks design with twelve treatments and three replications. These treatments along with a chemical fertilizer control treatment were also evaluated using a randomized complete blocks design with thirteen treatments and three replications. Studied traits were plant height, umbel no/ plant, biological and seed yield, essential oil content and essential oil yield. The results revealed that in organic system applying the third level of vermicompost combined with zeolite and PGPR inoculation significantly increased umbel no/ plant, essential oil content and essential oil yield in comparison with chemical system. Such findings were retrieved by other investigators on different medicinal plants such as Ocimum basilicum and Rosmarinus officinalis [1,2]. There was no significant difference between these nutrition systems on plant height, biological and seed yield, but in organic system these values were higher than chemical system. Generally the most favourable qualitative characteristics were obtained from organic system. In addition organic system could have environmental advantages when compared with chemical system.