To study the effect of organic and biofertilizers on the growth, yield and essential oil of Matricaria chamomilla L. a field experiment has been carried out in Iran during 2008. The factors were PGPR inoculation (inoculated and no inoculated), zeolite and Vermicompost. Studied characteristics were plant height, dry flower yield, essential oil content and yield. The results showed that Vermicompost levels had significant effects on all studied parameters. With increasing vermicompost levels, plant height, dry flower yield, essential oil content and yield were increased. The highest and the lowest of essential oil content were observed in 10 and 0 ton/ha Vermicompost levels, respectively. Also there were positive and synergistic interaction between factors Vermicompost combined with PGPR and Vermicompost combined with zeolite on dry flower yield. Zeolite also showed significant effects on mentioned traits as wich increasing zeolit amount, essential oil yield increased and there was significant difference between 0 and 9 ton/ha zeolite. The highest and the lowest of dry flower yield were obtained in inoculated and no inoculated PGPR. Consequently, it seems that the the organic cultivation of German chamomile can consider as an alternative system for conventional system in chamomile production.