Four species belonging to two subgenera of Iranian Allium species were chromosomally and karyotypically assessed, using squash technique and feulgen stain. Ploidy level of A. longivaginatum, A. hooshidaryae and A. remediorum (2n = 2x = 16) are reported for the first time. Results indicated that A. rotundum is tetraploid (2n=4x= 32). Two chromosome types (‘‘m’’, ‘‘sm’’) formed different karyotypic formulas. Mean chromosome length varied from 7.8 - 13.56 μm. The results show that Allium longivaginatum has the most asymmetrical karyotype.
