Aim. The purpose of this study was to investigate the effect of acute resistance exercise (RE), aerobic exercise (AE) and concurrent exercise (CE) on serum visfatin and insulin concentrations. Methods. Nine healthy male subjects (Mean ± SD; age, 24.2±3.6 yr; body mass index, 21.8±2.8 kg/m2) performed three exercise trials on separate days in a randomized balanced design. The three protocols were: 1) RE protocol which included three sets of 15 repetitions at 60% of one repetition maximum (1RM) for 7 exercises, 2) AE protocol included 50 min cycling at an intensity corresponding to 60% of HRmax on a cycle ergometer, 3) CE protocol encompassed two sets of 15 repetitions at 60% of 1RM for 7 exercises and 22 min cycling at 60% of HRmax. Three blood samples were collected before, immediately after exercise and after 30 min recovery and were analyzed for visfatin and insulin concentrations. Results. Serum visfatin and insulin levels were reduced significantly (P<0.05) in response to all exercise protocols, and remained unchanged after 30-min recovery. However, neither responses to exercise nor changes during recovery in visfatin and insulin were significantly different among the three exercise trials (P>0.05). Conclusion. It could be concluded that different acute exercise protocols reduce serum visfatin and insulin levels and that these responses are not related to type of exercise.