Purpose Exercise-induced free radical production and damage to cellular macromolecules have been previously documented. Therefore, the purposes of this study were to determine the effects of acute resistance exercise (RE) on the 8-iso PGF2a and 8-hydroxy-20-deoxyguanosine (8- OHdG), markers of lipids and DNA oxidation, and whether resistance training status influences these markers of oxidative stress. Methods Nine recreationally resistance trained (RT) and nine untrained (UT) men who did not have RE experience participated in this study. All participants performed a RE protocol, including four sets of bench press, leg press, seated bar shoulder press, arm curls, and lat pull down exercises at 80% 1RM load. Urine samples were collected at before (pre), after (post) and 3 h (3 h post) and 24 h after RE (24 h post) for measuring 8-iso PGF2a and 8-OHdG levels. Results Urinary 8-OHdG levels were significantly lower in the post and 24 h post RE in RT compared with UT. Significant increases in 8-OHdG levels were observed post, 3 h post, and 24 h post RE in relation to preexercise in UT. Also, 8-OHdG levels were significantly elevated at 3 h post and 24 h post REs as compared with preexercise in RT. There were no significant differences in 8-iso PGF2a levels between groups. Interestingly, 8-iso PGF2a levels were significantly decreased 3 h post as compared with post RE for both groups. Conclusions These data suggest that resistance training status of the participants partly prevented oxidative damage to DNA, while having a little impact on lipid peroxidation.
