Grapevine trunk diseases (GTDs) are destructive fungal diseases and economically important threat to grapevine industry worldwide. During a survey of vineyards in the Kurdistan Province, 233 fungal isolates were obtained that associated with various external and internal trunk diseases symptoms. Based on sequence data and morphological characteristics, 24 species belonging to 19 genera were characterized. Botryosphaeriaceae species were the most prevalent identifed fungi followed by Alternaria, Sporocadaceae, Phaeoacremonium, and Didymellaceae taxa. At the species level, Botryosphaeria doth- idea, Alternaria malorum, Phaeoacremonium aleophilum, Acremonium sclerotigenum, Alternaria chlamydosporigena, and Neoscytalidium dimidiatum were the most frequent identifed species. Acremonium sclerotigenum, A. chlamydosporigena, A. malorum, Juxtiphoma eupyrena, and Paecilomyces formosus are new records associated with GTDs around the world. Among the four pestalotia-like species identifed based on morphology and phylogenetic analyses of LSU, ITS, TEF1, and TUB2 sequence data, three species are newly described and introduced here as Seimatosporium marivanicum, Sporocadus kurdistanicus, and Xenoseimatosporium kurdistanicum. Furthermore, three new combinations are proposed, including Spo- rocadus corni, Spo. italicus, and Spo. pseudocorni. Pathogenicity of 15 species was studied on cultivars Rasha and Bidaneh Sefd in feld conditions. The pathogenicity of Neoscytalidium novahollandiae, B. dothidea, Ph. aleophilum, and Phaeomo- niella chlamydospora was confrmed in feld conditions.
