Dothiorella species with Dothidotthia te1eomorph are found on a wide range of woody hosts. During a survey in 2005-2007 some 28 isolates were obtained from different woody plants from several provinces of Iran. Isolation were made from single conidia or by directly plating out pieces of diseased tissue after surface sterilization in 96% ethanol for 3 min. Identification of the isolates were achieved based on conidial characteristics. Sporulation was enhanced by placing autoclaved pine needles on 2% water agar. The plates were incubated at 25°C under near-UV light with a 12/12 hour photoperiod for 3-6 wk. Sections through pycnidia of some isolates were made with Leica CM100 cryostat. Colony features were determined on MEA and PDA at 25°C. In this survey, four species including Dothiorella sarmentorum, Do. cf. viticola, Dothiorella sp.1 and Dothiorella sp.2 were identified. All species are reported here for the first time from Iran.
