Prostigmata is the larger of the two suborders of the order Trombidiformes within the mite superorder Acariformes. Members of the Bdelloidea and Raphignathoidea are important predatory mites in suborder Prostigmata. They are active predators of small arthropods and have high potential as biological control agents in agricultural ecosystems. To identify of the superfamilies Bdelloidea and Raphignathoidea, sampling was taken from soil and tree foliages of orchards during 2020-2021 in different places of Damghan city, Semnan province. Collected specimens were extracted using the Berlese-Tullgren funnel and put into AGA solution. Mites were cleared in lactic acid and then microscopic slides were mounted in Hoyer’s medium. During this study, a total number of 18 species from 14 genera and 6 families were collected and identified. Among them, Cyta kreiteri Barbar & Ueckermann, 2017 were recorded for the second time from Iran. Also, Bdella muscorum (Ewing, 1909) was the dominant predator species in this study. The identified species are as bellow list: I. Bdelloidea. Bdellidae: Bdella muscorum Ewing, 1909; Biscirus iranensis Paktinat-Saeej & Bagheri, 2015; Cyta latirostris (Hermann, 1804); C. coerulipes (Dugès, 1834); C. kreiteri Barbar & Ueckermann, 2017; Odontoscirus meridionalis (Thor, 1931); O. mazandaranensis Paktinat-Saeij, Bagheri & Hernandes, 2016; Spinibdella cronini (Baker & Balock, 1944); Cunaxidae: Cunaxa gordeevae Sergeyenko, 2009; C. grobleri Den Heyer, 1979; Cunaxoides sp.; Armascirus hastus Shiba, 1986. II. Raphignathoidea. Caligonellidae: Caligonella sp.; Cryptognathidae: Cryptognathus lagena Kramer, 1879; Favognathus mirazii Khanjani & Ueckermann, 2008; Raphignathidae: Raphignathus gracilis (Rack, 1962); Stigmaeidae: Eustigmaeus segnis (Koch, 1836); Cheylostigmaeus sp.
