2024 : 10 : 6

Ghader Mirzaghaderi

Academic rank: Professor
ORCID:
Education: PhD.
ScopusId: 24335609700
HIndex:
Faculty: Faculty of Agriculture
Address: Department of Agronomy and Plant Breeding - College of Agriculture - University of Kurdistan - P.O. Box: 416 - Sanandaj - Iran
Phone:

Research

Title
Repetitive DNA landscape in essential A and supernumerary B chromosomes of Festuca pratensis Huds
Type
JournalPaper
Keywords
Tandem repeats, Satellite DNA, Next Generation Sequencing
Year
2019
Journal Scientific Reports
DOI
Researchers Rahman Ebrahimzadegan ، Andreas Houben ، Ghader Mirzaghaderi

Abstract

Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (−B) and B chromosome containing (+B) individuals of F. pratensis. 61% of the nuclear genome is composed of repetitive sequences. 43.1% of the genome are transposons of which DNA transposons and retrotransposons made up 2.3% and 40.8%, respectively. LTR retrotransposons are the most abundant mobile elements and contribute to 40.7% of the genome and divided into Ty3-gypsy and Ty1-copia super families with 32.97% and 7.78% of the genome, respectively. Eighteen different satellite repeats were identified making up 3.9% of the genome. Five satellite repeats were used as cytological markers for chromosome identification and genome analysis in the genus Festuca. Four satellite repeats were identified on B chromosomes among which Fp-Sat48 and Fp-Sat253 were specific to the B chromosome of F. pratensis.