2024 : 4 : 14

Ghader Mirzaghaderi

Academic rank: Professor
ORCID:
Education: PhD.
ScopusId: 24335609700
Faculty: Faculty of Agriculture
Address: Department of Agronomy and Plant Breeding - College of Agriculture - University of Kurdistan - P.O. Box: 416 - Sanandaj - Iran
Phone:

Research

Title
A Robertsonian translocation from Thinopyrum bessarabicum into bread wheat confers high iron and zinc contents
Type
JournalPaper
Keywords
Biofortification, grain Grain Fe and Zn, PLUG marker, Triticum aestivum
Year
2016
Journal PLANT BREEDING
DOI
Researchers Somayeh Ardalani ، Ghader Mirzaghaderi ، Hedieh Badakhshan

Abstract

Development of wheat–alien translocation lines has facilitated practical utilization of alien species in wheat improvement. The production of a compensating Triticum aestivum-Thinopyrum bessarabicum whole arm Robertsonian translocation (RobT) involving chromosomes 6D of wheat and 6Eb of Th. bessarabicum (2n = 2x = 14, EbEb) through the mechanism of centric breakage-fusion is reported here. An F2 population was derived from plants double-monosomic for chromosome 6D and 6Eb from crosses between a DS6Eb(6D) substitution line and bread wheat cultivar Roushan (2n = 6x = 42, AABBDD) as female parent. Eighty F2 genotypes (L1 to L80) were screened for chromosome composition. Three PCR-based Landmark Unique Gene (PLUG) markers specific to chromosomes 6D and 6Eb were used for screening the F2 plants. One plant with a T6EbS.6DL centric fusion (RobT) was identified. A homozygous translocation line with full fertility was recovered among F3 families and verified with genomic in situ hybridization (GISH). Grain micronutrient analysis showed that the DS6Eb(6D) substitution line and T6EbS.6DL stock have higher Fe and Zn contents than the recipient wheat cultivar Roushan.