The objective of the present study was to produce compensating wheat–Thinopyrum bessarabicum whole arm Robertsonian translocations (RobTs) involving chromosomes 2B of wheat and 2Eb of Th. bessarabicum through the mechanism of centric breakage-fusion. F2 population from crosses between 2Eb (2B) substitution line and bread wheat 'Roushan' (2n = 6x = 42, AABBDD) as female parent were made and forty one BC1F2 lines (L1 to L41) were screened for their chromosome composition. Seven wheat-Th. bessarabicum (Eb genome) Robertsonian translocations were identified among the studied lines using PCR-based Landmark Unique Gene (PLUG) markers. Homozygous translocations (T2EbS.2BL) with good plant vigor and full fertility were selected from F3 families. Seed length was significantly higher in both translocations relative to their wheat parent. The T2EbS.2BL stock is cytogenetically stable, and may be useful in wheat improvement.
