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Ghader Mirzaghaderi

Academic rank: Professor
ORCID:
Education: PhD.
ScopusId: 24335609700
Faculty: Faculty of Agriculture
Address: Department of Agronomy and Plant Breeding - College of Agriculture - University of Kurdistan - P.O. Box: 416 - Sanandaj - Iran
Phone:

Research

Title
A simple metaphase chromosome preparation from meristematic root tip cells of wheat for karyotyping or in situ hybridizationجدید
Type
JournalPaper
Keywords
Fluorescence in situ hybridization (FISH), Karyotyping, Squashing method, Wheat
Year
2010
Journal African Journal of Biotechnology
DOI
Researchers Ghader Mirzaghaderi

Abstract

In this paper a simple and efficient metaphase chromosome preparation method from wheat has been described which also can be applied on most of other plants. Lack of using hydrolyzing chemicals such as hydrochloric acid in this method made the samples suitable for C-banding and in situ hybridization as well. Squeezing out the meristematic cells from the root tip region on to the surface of the slide is the main step of the described method, leading to get monolayer and clean preparations. In order to arrest cells in metaphase, colchicines, α-monobromonaphthalene, ice cold water, colchicines-ice cold water and α-monobromonaphthalene-ice cold water were compared to each other. Ice water pretreatment resulted in the highest metaphase index (up to 20% in some cultivars). Some preparations were used for in situ hybridization. The described method can be easily applied by beginner plant cytogeneticians.