برزان بهرامی کمانگر

In order to induce meiosis gynogenesis in rainbow trout (Oncorhynchus mykiss), spermatozoa genome was destroyed by Uv irradiation (2887 J.J w/cm2) and after fertilization with intact ovum, diploidy was returned by applying a thermal shock (27 + O.5°c) for 10 minutes. Thermal shock was applied at 30 and 40 minutes after fertilization. Uv-irradiation was supplied from a germicidal lamp (30w) with maximum intensity at 245nm. Before irradiation, sperm was diluted with two kinds of solutions at a ratio of 1:4 and then subjected to 1,3,5,8 and 15 minutes of irradiation. Statistical design for comprising effects on 3 different treatment groups were observed as split split plot on gynogenesis and split plot on haploid and randomized complete block on control. Golden color in rainbow trout was used as a marker for distinguishing gynogenetic offspring. Furthemore, for confirmed diploidy chromosome slides were prepared. The best performance was achieved in treatment of 8 minute irradiation and applied thermal shock of 30 minutes after fertilization, but there was no significant difference between the two solutions (P>0.O5). The mean percentage gynogenesis was 60.61 % in the treatments of three replications. Neverthless, 100% gynogenesis was achieved from 8 minute irradiation in the second replication.