Different physical factors in a hospital setting can have qualitative and quantitative effects on hospitalization of patients. In other words, the proper hospital is not only faced with medicinal needs, but also with the movemental, social, evolutionary and emotional needs of patients, should also be considered in the architectural design. Hospitalization is a stressful experience for children that can lead to emotional isolation, various regressive behaviors, prolonged crying, disturbed sleep patterns and a variety of devastating conditions that become angry at being exposed to such attacks. Accordingly, the present study aimed to identify environmental factors affecting children’s morale in stress reduction in hospital environment besides physical and functional analysis of six different children’s hospital samples in the world. In this paper, systematic review method was used for initial recognition and also descriptive-comparative method was used to express the effective features in the design of case samples architecture. The results of the studies indicate that environmental and physical factors have a significant impact on reducing children’s stress in a hospital environment, at the same time paying attention to the attractiveness, readability and simplicity of functional spaces for them.