Silver nanoparticles (AgNPs) have been increasingly used as antimicrobial and disinfectant. However, intestinal model studies have shown that AgNPs induce oxidative stress. Hence, this study aims to investigate the effects of dietary supplemental zinc (Zn) and vitamin E (VE, α-tocopherol acetate) on attenuating AgNP-induced intestinal oxidative stress in broiler chickens. The chickens were divided into two groups as follows: 1. control group fed with a corn–soybean meal basal diet and 2. Nano group, received drinking water containing 1000 mg/kg AgNPs. All the Nano-exposed birds were divided into six dietary treatment groups, namely, the basal diets supplemented with 1) 60 mg/kg Zn as ZnSO4, 2) 120 mg/kg Zn, 3) 100 mg/kg VE, 4) 200 mg/kg VE, 5) 60 mg/kg Zn and 100 mg/kg VE, and 6) 120 mg/kg Zn and 200 mg/kg VE. Results showed that the AgNPs significantly reduced the body weights of the broilers after 42 days of oral administration of AgNPs (P<0.05), and this effect was not alleviated by any of the dietary treatments. The activity of superoxide dismutase (CuZn-SOD) increased in all the AgNP-treated birds (P<0.05); however, CuZn-SOD did not increase in birds fed with basal diet supplemented with 200 mg/kg VE. In this treatment, the VE exerted an antioxidant effect to prevent the activation of the CuZn-SOD enzyme. Furthermore, supplementing Zn increased the activities of catalase and glutathione peroxidase in the jejunal mucosa (P<0.05), which were accompanied with increased malondialdehyde levels (P<0.05) in the broilers. AgNP exposure resulted in a significant mRNA up-regulation of toll-like receptor 4 (TLR4) and TLR2-1 in the jejunal mucosa (P<0.05). However, supplemental ZnVE did not reduce TLRs mRNA expression, except for the diminished TLR2-1 mRNA levels in birds fed with basal diet supplemented with 120 mg/kg Zn and 200 mg/kg VE. We concluded that although dietary Zn and VE supplementation did not attenuate growth depression effect of AgNP, however attenuate inte
