Electrogenerated chemiluminescence (also called electrochemiluminescence and abbreviated ECL) has attracted much attention in various fields of analysis due to the potential remarkably high sensitivity, extremely wide dynamic range and excellent controllability. Electrochemiluminescence biosensor, by taking the advantage of the selectivity of the biological recognition elements and the high sensitivity of ECL technique was applied as a powerful analytical device for ultrasensitive detection of biomolecule. In this review, we summarize the latest sensing applications of ECL bioanalysis in the field of bio affinity ECL sensors including aptasensors, immunoassays and DNA analysis, cytosensor, molecularly imprinted sensors, ECL resonance energy transfer and ratiometric biosensors and give future perspectives for new developments in ECL analytical technology. Furthermore, the results herein discussed would demonstrate that the use of nanomaterials with unique chemical and physical properties in the ECL biosensing systems is one of the most interesting research lines for the development of ultrasensitive electrochemiluminescence biosensors. In addition, ECL based sensing assays for clinical samples analysis and medical diagnostics and developing of immunosensors, aptasensors and cytosensor for this purpose is also highlighted.
