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Abdollah Salimi

Abdollah Salimi

Academic rank: Professor
ORCID:
Education: PhD.
ScopusId: 57198900488
Faculty: Faculty of Science
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Research

Title
Preparation and characterization of laccases immobilized on magnetic nanoparticles and their application as a recyclable nanobiocatalyst for the aerobic oxidation of alcohols in the presence of TEMPO
Type
JournalPaper
Keywords
laccases, magnetic nanoparticle ,nanobiocatalyst, immobilize, oxidation, alcohols, TEMPO
Year
2016
Journal RSC advances
DOI
Researchers Shamila Rouhani ، Amin Rostami ، Abdollah Salimi

Abstract

Laccase from Trametes versicolor was immobilized on a modified magnetic nanoparticle (MNP-Laccase) through a covalent attachment method. The morphology, structure, magnetic property and chemical composition of the immobilized laccase (MNP-Laccase) were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), alternating gradient force magnetometry (AGFM), energy-dispersive X-ray spectroscopy (EDX) and thermogravimetric analysis (TGA) techniques. The MNPs-Laccase retained the activity and exhibited higher resistance to pH and temperature changes. We have used MNPs-Laccase as a magnetically recoverable nanobiocatalyst for the mild, environmentally friendly and selective aerobic oxidation of benzylic and allylic alcohols to corresponding aldehydes in the presence of TEMPO as a redox mediator at room temperature. The magnetic nanobiocatalyst was easily and rapidly recovered by applying an external magnet device and reused up to 6 reaction runs without considerable loss of reactivity.