In this study, the preparation of an integrated modified electrode based on the covalent attachment of glucose dehydrogenase (GDH) enzyme and safranin O toamine-derivative multi walled carbon nanotubes (MWCNTs-NH2) modified glassy carbon(GC)electrodeusingG2.5-carboxylated PAMAM dendrimer (Den) as linking a gentisreported. The obtained results indicated that the proposed system haseffective bioelectrocatalytic activity toward glucose oxidation at 100 mV with on setpotential of −130mV(vs.Ag/ AgCl). The performance of the prepared hybrid system of GC/MWCNTs-NH2/Den/GDH/Safranin as anode in a membr an elessenzyme-basedglucose/O2 biofuel cell is further evaluated. The biocathodeinthis system was composed of bilirubinoxidase (BOX) enzyme immobilizedontoa bilirubin modified carbon nanotube GC electrode. Immobilized BOX on to CNTs/bilirubinnot only show direct electron transfer but also it has excellent electro catalytic activity toward oxygen reduction at appositive potential of 610 mV. The open circuit voltage of the cell was 590 mV. The maximum current density was 0.5 m Acm−2, while maximum power density of 108 μWcm−2 was achieved at voltage of 330 mV. The immobilizedenzymes in anodeandcathodear every stable and output power of the BFC is approximately constant after 12 h continues operation.
