Iron is an essential element which is used by body for the production of hemoglobin which absorbs oxygen and distributes it to the tissues Non—heme iron is stored in the body by the protein ferritin and transported by the protein transferrin both of which are not saturated with Fe(III) . In cases of iron overload the natural storage and transport proteins are overwhelmed and the iron spills over into other tissues and organs such as the muscle, spleen and liver[l]. As it accumulates, difficulties arise because of the toxicity of metal ions[l]. Thus, the determination of trace amounts of iron from different of matrices is of great importance. Numerous methods, such as spectrophotometric[2-3], potentiometric titration, flame elctrothermall atomic absorption spectrophotometric, flow- injection and photoacousti methods have been used for the determination of iron species.
