The inclusion of DDGS in poultry diets is limited due the reduced content of starch and high levels of Non Starch Polysaccharides (NSP). An improvement on the nutrient availability through the use of exogenous enzymes represents an alternative to increase its utilization. Rovabio Max is a preparation containing xylanases, β-glucanases, pectinases, mannanases, phytase and α-galactosidase. A study was conducted to evaluate the use of Rovabio Max on utilization of diets containing high levels of DDGS. The experimental design consisted of a 2 × 4 factorial arrangement. Two isocaloric (ME = 3020 kcal/kg) basal diets were formulated, one with no DDGS and the second with 30% DDGS of known composition. Chromic oxide was used as an indigestible marker. Aliquots of the 2 basal diets were supplemented with no enzyme or 3 levels of Rovabio: The recommended level (1X), twice (2X) and 4 times (4X) the recommended level. One hundred and 90 2 male chicks of a commercial strain (Cobb 500) were fed a common nutritionally complete diet to 18 d at which time they were placed on the study. Each experimental diet was fed to 4 pen replicates of 6 male chicks in wire floor battery cages. After a 5-d adaptation period, excreta samples were collected and freezedried to determine GE, AME and N retention. Birds were weighed and feed consumption determined. The ANOVA considered DDGS level, enzyme, and interaction. No significant (P > 0.05) effect of Rovabio, DDGS and their interactions was found on body weight, feed intake, feed conversion and mortality. No effect of the enzyme was found for GE, N in excreta, N retention, AME and AMEn. No interaction between DDGS and Rovabio was found for nitrogen and energy utilization. The inclusion of 30% DDGS in the diet significantly (P < 0.05) increased the GE and N in excreta with no effect on AME and AMEn values. The increased concentration of nutrients in excreta deserves consideration due to environmental implications.