Broilers performance in the end of rearing may be influenced by post hatch feeding and the duration of feeding the starter and grower diets. Those conditions also affect some biochemical indices of their blood, which in turn may affect their feeding and growth rate finally. This study was conducted with 720, Ross 308 broiler chickens to evaluate the influence of delayed access to feed and water in neonatal period. Four experiments were conducted to determine the effects of post hatch feeding time and starter phase length on blood level of glucose, cholesterol, triglycerides and triiodothyronine of broiler chickens. Three fasting regiments were utilized in each experiment: 1) No fasting 2) 16 hours and 32 hours fasting. Starter phase for each experimental group were 11, 16, and 21 days. Blood samples had taken at days 3, 21 and 42 after hatching. Sera were analyzed with auto analyzer for above mentioned parameters. Serum glucose value for the test birds was high in accordance to length of deprivation time. Levels of cholesterol and triglycerides have not affected meaningfully but serum triiodothyronine (T3) concentration, which may mediate some of the intestinal effects of feed deprivation were depressed in broilers without access to feed.