Dissemination of nanomaterials (NMs) in air, water or soil can create risks for the environment, and when NMs exist together in aquatic environments, they can interact with each other through physical and chemical processes. Humans may also be exposed to NMs through seafood consumption. Brine shrimp (Artemia salina) is widely used in biological studies and is a well-known organism in the nanoecotoxicological field. In this study, A. salina nauplius were exposed to different concentrations of silver nanoparticles (AgNPs) and silver ions (Ag+) individually or in combination with different concentrations (1, 10 and 100 mg/L) of polystyrene nanoplastics (NPs). Toxicity tests were conducted in accordance with ISO/TS 20787 and effective concentrations were calculated using Probit analysis program. Also the interaction behavior of these materials was investigated in the exposure media. The results showed that the median effective concentrations (EC50s) of both AgNPs and Ag+ were higher in the presence of polystyrene NPs. In other words, polystyrene NPs were reduced the toxicity of silver compounds. More studies are necessary to investigate the scientific reasons of this phenomenon in the future.
