Chrysanthemum morifolium within Asteracea family which is one of the most important cut flower and pot species. Callus tissue is genetically unstable and regeneration via this tissue is associated with the occurrence of somaclonal variation or chimera structure. The use of ray florets and leafy tissues for the in vitro propagation of C. morifolium for shoot regeneration (Direct and indirect) or somatic embryogenesis is of great importance for the mutagenic breeding programs and genetic transformtion of this species. Cytokinin and auxin hormones have been effective in shoot regeneration and creating calluses in C. morifolium species. The ability to regenerate shoots from callus is essential for establishing a successful plant culture system as well as in vitro mutagenesis. In this mini review, induction of embryogenic callus and direct organogenesis and importance of these methods in mutagenesis and genetic transformation are described
