Carbohydrates are an essential part of in vitro culture medium and have effect on grape shoot regeneration of shoot formation in current study two explants (node and shoot tip) of two grape cultivars (Khoshnave and Farkhi) in medium containg different fructose concentration (15, 20, 30 and 40 gL-1) was study. some parameters such as main shoot length, leave number of adventitious shoot were evaluated. The highest and lowest shoot length were observed in 30 and 15 gL-1 of fructose respectively. Although the highest leaf number were observed in 40 gL -1 of fructose but This concentration induced high levels sample vitrification, shoot tip explants produced longer shoot then node explants but the number of adventitious shoot was higher in node explants.
