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Title
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Investigation of crystallographic structure, in vitro cytotoxicity and DNA interaction of two La(III) and Ce(IV) complexes containing dipicolinic acid and 4-dimethylaminopyridine
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Type
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JournalPaper
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Keywords
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Lanthanide complexes Cytotoxicity DNA interaction Calf thymus deoxyribonucleic acid Molecular docking
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Abstract
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The compound, [dmpH]2[pydc] (L) as ligand was synthesized by the proton-transfer reaction of pyridine- 2,6-dicarboxylic acid (pydcH2) with 4-dimethylaminopyridine (dmap). The new coordination complexes of [dmpH]2[La2(pydc)4(H2O)4]2H2O (1) and [dmpH]2[Ce(pydc)3]2H2O (2) were prepared from the reaction of the ligand with La(NO3)36H2O and Ce(SO4)24H2O metal salts, respectively. All structures were characterized by X-ray crystallography. Cytotoxicity of compounds was tested under MTT method, against HT29 (a human colon adenocarcinoma), HepG2 (a human liver hepatocellular carcinoma) and HL60 (a human lymphocyte) cell lines. Complex (1) with IC50 values of about 1–100 lM was more active than other compounds on all three cell lines. The strongest anti-proliferative effect was observed for (1) toward HL60 cell line with IC50 value equal to 1 lM. High cytotoxicity of complexes indicated that they can be further evaluated for cancer treatment in the next set. UV–Vis absorption spectroscopy, emission titration and viscosity were employed to investigate the mode of interaction between (L), (1) and (2) with calf-thymus DNA. All studies represented that (L), (1) and (2) interact with CT-DNA via intercalation mode. A direct correlation was recognized between inhibitory and DNA interaction potent of the compounds. Also, the results of molecular docking were in agreement with obtained experimental data of DNA interaction.
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Researchers
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Andya NEmati (Not In First Six Researchers), Giuseppe Bruno (Not In First Six Researchers), Hadi Amiri Rudbari (Not In First Six Researchers), Mohabbat Ansari (Not In First Six Researchers), Mohammad Ghadermazi (Not In First Six Researchers), Saba Zendehcheshm (Not In First Six Researchers), Mohammad Mahdavi (Fifth Researcher), aynaz golabi (Fourth Researcher), Nahid Shahabadi (Third Researcher), Sara Abdolmaaleki (Second Researcher), Hadi Adibi (First Researcher)
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