In this article, ITS sequences of 34 species in Bulbophyllum genus were researched. Alignment between ITS sequences from the species was performed, and discovered that average percentage sequence divergence (uncorrected p distance) within Bulbophyllum species was 9.87% and maximum in-group ITS divergence was 13.68% (between B. membranifolium with B. sulcatum). Molecular systematic analysis of ITS was revealed that, 34 species of Bulbophyllum were monophyletic and they were divided into six groups, which is partly inconsistent with the viewpoint of morphological classification of Bulbophyllum in Peninsular Malaysia.
